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KMID : 0385520160290030105
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Analytical Science & Technology
2016 Volume.29 No. 3 p.105 ~ p.113
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Effect of functional group on activity and stability of lipase immobilized on silica-coated magnetite nanoparticles with different functional group
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Lee Hye-Rin
Ha Sung-Ho
Kim Moon-Il
Hong Sang-Eun
Choi Jae-yeong
Yoon Kuk-Ro
Lee Seung-Ho
Kim Young-Min
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Abstract
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The present study investigated the immobilization of lipases on silica nanoparticles and silica-coated magnetite nanoparticles as supports with a functional group to enhance the stability of lipase. The influence of functional groups, such as the epoxy group and the amine group, on the activity and stability of immobilized lipase was also studied. The epoxy group and the amino group were introduced onto the surface of nanoparticles by glycidyl methacrylate and aminopropyl triethoxysilane, respectively. Immobilized Candida rugosa lipase on silica nanoparticles and silica-coated magnetite nanoparticles with a functional group showed slightly lower initial enzyme activities than free enzyme; however, the immobilized Candida rugosa lipase retained over 92 % of the initial activity, even after 3 times reuse. Lipase was also immobilized on the silica-coated magnetite nanoparticles by cross-linked enzyme aggregate (CLEA) using glutaraldehyde and covalent binding, respectively, were also studied. Immobilized Candida rugosa lipase on silica nanoparticles and silica-coated magnetite nanoparticles by CLEA and covalent binding showed higher enzyme activities than free enzyme, while immobilized Candida rugosa lipase retained over 73 % of the initial activity after 5 times reuse.
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KEYWORD
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nanoparticle, silica-coated magnetite nanoparticles, lipase, immobilization, activity, stability,
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